Column:

Target SB-C18, 5 µm, 100 Å, 4.6 × 250 mm

Mobile phase:

10 mM KH2PO4 (pH 3.0) : ACN = 70 : 30 (v/v)

Flow rate:

1.0 mL/min

Temperature:

Ambient

Detection:

UV 220 nm

Injection Volume:

20 µL

Sample:

1. Ractopamine （莱克多巴胺）
2. Clenbuterol （克伦特罗）

Column:

Target SB-C18 5 µm, 100 Å, 4.6 × 150 mm

Mobile phase:

ACN : 0.05%SDS (containing 0.1%H3PO4) = 48 : 52 (v/v)

Flow rate:

1.0 mL/min

Temperature:

Ambient

Detection:

UV 265 nm

Injection Volume:

10 µL

Sample:

1. Berberine hydrochloride （盐酸小蘗碱）

Column:

Target SB-C18, 5 µm, 100 Å, 4.6 x 250 mm

Mobile phase:

Time(min): 
B%:

A: CH3OH   
B: H2O ( 0.05% TFA )
0     4   15   21  25  30
78  78  60  60  78  78

Flow rate:

1.0 mL/min

Temperature:

35 ℃

Detection:

UV 278 nm

Injection volume:

10 µL

Sample:

1 mg/mL Catechins （儿茶素） in water